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Zhong Yao Cai. 2013 Jun;36(6):967-71.

[Effect of emodin lipid nano-microbubble on MAPK signal pathway and inflammation cytokine in AT-II cells by mechanical stretch].

[Article in Chinese]

Author information

1
Guangdong Food and Drug Vocationl College, Guangzhou 510520, China. jiangyn@gdyzy.edu.cn
2
National Key Laboratory of Respiratory Disease/First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, China.
3
Guangdong Food and Drug Vocationl College, Guangzhou 510520, China.

Abstract

OBJECTIVE:

To investigate the effect of emodin lipid nano-microbubble on MAPK signaling pathway and the level of inflammation cytokine in AT-II cells induced by mechanical stretch and its mechanism.

METHODS:

Emodin nanofibers, prepared by electrospinning with lecithin and PVP as carrier, were put into a seal bottle full of perfluoropropane, after they mixed with water and turned into self-assembled nano-microbubble. AT-II cells, separated and purified from primary rat AT-II cells, suffered 20% mechanical stretch for 4, 8, 16, 24, 48 h, and its protein expressions of p-P38/P38, p-ERK/ERK, p-JNK/JNK and the inflammation cytokine release levels of TNF-alpha, IL-1beta, IL-6 were detected by Western-Blot and ELISA. And further oberved the intervention effect of emodin lipid nano-microbubble on VILI.

RESULTS:

The protein expressions of p-P38, p-ERK, p-JNK were significantly increased in AT-II cells induced by mechanical stretch, and continuously evaluated from the 4-16 h, but the protein expressions of p38, ERK, JNK had no significant difference. The release levels of TNF-alpha, IL-1beta, IL-6 in AT-II cells had no change during 8 h, and they were gradually increased significantly in followed 16 h. In AT-II cells induced by mechanical stretch, due to intervention effect of emodin lipid nano-microbubble, the the protein expressions of p-P38, p-ERK, p-JNK and the inflammation cytokine release levels of TNF-alpha, IL-1beta, IL-6 were significantly decreased.

CONCLUSION:

Emodin lipid nano-microbubble shows protective effect on AT-II cells induced by mechanical stretch (VILI), and its mechanism may be related to down-regulation of protein expression of MAPK signaling pathway to regulate the release levels of inflammatory cytokine.

PMID:
24380287
[Indexed for MEDLINE]
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