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Biomed Res Int. 2013;2013:412745. doi: 10.1155/2013/412745. Epub 2013 Nov 27.

The study on biocompatibility of porous nHA/PLGA composite scaffolds for tissue engineering with rabbit chondrocytes in vitro.

Author information

1
Guangzhou Medical College, Guangzhou, Guangdong 510182, China ; Department of Orthopedics, Second People's Hospital of Shenzhen, Sungang West Road, Futian District, Shenzhen, Guangdong 518035, China ; Shenzhen Key Laboratory of Tissue Engineering, Shenzhen, Guangdong 518035, China.
2
Department of Orthopedics, Second People's Hospital of Shenzhen, Sungang West Road, Futian District, Shenzhen, Guangdong 518035, China ; Shenzhen Key Laboratory of Tissue Engineering, Shenzhen, Guangdong 518035, China.
3
Biomechanics and Medical Information Institute, Beijing University of Technology, Beijing 100022, China.

Abstract

OBJECTIVE:

To examine the biocompatibility of a novel nanohydroxyapatite/poly[lactic-co-glycolic acid] (nHA/PLGA) composite and evaluate its feasibility as a scaffold for cartilage tissue engineering.

METHODS:

Chondrocytes of fetal rabbit were cultured with nHA/PLGA scaffold in vitro and the cell viability was assessed by MTT assay first. Cells adhering to nHA/PLGA scaffold were then observed by inverted microscope and scanning electron microscope (SEM). The cell cycle profile was analyzed by flow cytometry.

RESULTS:

The viability of the chondrocytes on the scaffold was not affected by nHA/PLGA comparing with the control group as it was shown by MTT assay. Cells on the surface and in the pores of the scaffold increased in a time-dependent manner. Results obtained from flow cytometry showed that there was no significant difference in cell cycle profiles between the coculture group and control (P > 0.05).

CONCLUSION:

The porous nHA/PLGA composite scaffold is a biocompatible and good kind of scaffold for cartilage tissue engineering.

PMID:
24380082
PMCID:
PMC3860085
DOI:
10.1155/2013/412745
[Indexed for MEDLINE]
Free PMC Article
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