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J Biol Chem. 2014 Feb 21;289(8):4815-26. doi: 10.1074/jbc.M113.518480. Epub 2013 Dec 30.

Coordinated regulation of serum- and glucocorticoid-inducible kinase 3 by a C-terminal hydrophobic motif and Hsp90-Cdc37 chaperone complex.

Author information

1
From the Department of Cancer Biology, Beckman Research Institute of the City of Hope, Duarte, California 91010 and.

Abstract

Serum- and glucocorticoid-inducible kinase 3 (SGK3) mediates a variety of cellular processes including membrane transport, cell proliferation, and survival, and it has been implicated in Akt-independent signaling downstream of oncogenic PIK3CA mutations (activating mutations in the α catalytic subunit of PI3K) in human cancers. However, the regulation of SGK3 is poorly understood. Here we report that SGK3 stability and kinase activation are regulated by the Hsp90-Cdc37 chaperone complex. Hsp90-Cdc37 associates with the kinase domain of SGK3 and acts in concert with a C-terminal hydrophobic motif of SGK3 to prevent Hsp70 association and ubiquitin ligase CHIP (C terminus of Hsc70-interacting protein)-mediated degradation. Phosphorylation of hydrophobic motif triggers release of Cdc37 and concomitant association of 3-phosphoinositide dependent kinase 1 (PDK1) to activate SGK3. Our study provides new insights into regulation of SGK3 stability and activation and the rationale for application of Hsp90 inhibitors in treating SGK3-dependent cancers.

KEYWORDS:

CHIP; Cdc37; Hsp90; Hydrophobic Motif; Molecular Chaperone; PI 3-Kinase (PI3K); Protein Stability; SGK3; Ubiquitination

PMID:
24379398
PMCID:
PMC3931044
DOI:
10.1074/jbc.M113.518480
[Indexed for MEDLINE]
Free PMC Article

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