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Asian Pac J Cancer Prev. 2013;14(11):6945-8.

Diosgenin inhibits hTERT gene expression in the A549 lung cancer cell line.

Author information

1
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran E-mail : rahmati_bio@yahoo.com.

Abstract

BACKGROUND:

Diosgenin, a steroidal saponin from a therapeutic herb, fenugreek (Trigonellafoenum-graceum L.), has been recognized to have anticancer properties. Telomerase activity is not detected in typical healthy cells, while in cancer cell telomerase expression is reactivated, therefore providing a promising cancer therapeutic target.

MATERIALS AND METHODS:

We studied the inhibitory effect of diosgenin on human telomerase reverse transcriptase gene (hTERT) expression which is critical for telomerase activity. MTT- assays and qRT-PCR analysis were conducted to assess cytotoxicity and hTERT gene expression inhibition effects, respectively.

RESULTS:

MTT results showed that IC50 values for 24, 48 and 72h after treatment were 47, 44 and 43μM, respectively. Culturing cells with diosgenin treatment caused down-regulation of hTERT expression.

DISCUSSION:

These results show that diosgenin inhibits telomerase activity by down-regulation of hTERT gene expression in the A549 lung cancer cell line.

PMID:
24377630
[Indexed for MEDLINE]
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