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Ann Neurol. 2014 Feb;75(2):266-76. doi: 10.1002/ana.24088. Epub 2014 Feb 20.

In multiple sclerosis, oligoclonal bands connect to peripheral B-cell responses.

Author information

1
Departments of Neurology, University of California, San Francisco, San Francisco, CA.

Abstract

OBJECTIVE:

To determine to what extent oligoclonal band (OCB) specificities are clonally interrelated and to what degree they are associated with corresponding B-cell responses in the peripheral blood (PB) of multiple sclerosis (MS) patients.

METHODS:

Mass-spectrometric proteomic analysis of isoelectric focused (IEF) cerebrospinal fluid (CSF) immunoglobulin G (IgG) was used in combination with next-generation deep-immune repertoire sequencing of PB and CSF IgG heavy chain variable regions from MS patients.

RESULTS:

We find evidence for ongoing stimulation and maturation to antibody-expressing B cells to occur primarily inside the central nervous system (CNS) compartment. B cells participating in OCB production can also be identified in PB; these cells appear to migrate across the blood-brain barrier and may also undergo further antigen stimulation in the periphery. In individual patients, different bands comprising OCBs are clonally related.

INTERPRETATION:

Our data provide a high-resolution molecular analysis of OCBs and strongly support the concept that OCBs are not merely the terminal result of a targeted immune response in MS but represent a component of active B cell immunity that is dynamically supported on both sides of the blood-brain barrier.

PMID:
24375699
PMCID:
PMC3961546
DOI:
10.1002/ana.24088
[Indexed for MEDLINE]
Free PMC Article
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