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Protein Sci. 2014 Mar;23(3):284-8. doi: 10.1002/pro.2413.

Signatures of n→π* interactions in proteins.

Author information

1
Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin.

Abstract

The folding of proteins is directed by a variety of interactions, including hydrogen bonding, electrostatics, van der Waals' interactions, and the hydrophobic effect. We have argued previously that an n→π* interaction between carbonyl groups be added to this list. In an n→π* interaction, the lone pair (n) of one carbonyl oxygen overlaps with the π* antibonding orbital of another carbonyl group. The tendency of backbone carbonyl groups in proteins to engage in this interaction has consequences for the structures of folded proteins that we unveil herein. First, we employ density functional theory to demonstrate that the n→π* interaction causes the carbonyl carbon to deviate from planarity. Then, we detect this signature of the n→π* interaction in high-resolution structures of proteins. Finally, we demonstrate through natural population analysis that the n→π* interaction causes polarization of the electron density in carbonyl groups and detect that polarization in the electron density map of cholesterol oxidase, further validating the existence of n→π* interactions. We conclude that the n→π* interaction is operative in folded proteins.

KEYWORDS:

Bürgi-Dunitz trajectory; electron density; hyperconjugation; pyramidalization; stereoelectronic effect

PMID:
24375625
PMCID:
PMC3945836
DOI:
10.1002/pro.2413
[Indexed for MEDLINE]
Free PMC Article

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