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Genetics. 2014 Mar;196(3):615-23. doi: 10.1534/genetics.113.159483. Epub 2013 Dec 27.

Large-scale mapping of transposable element insertion sites using digital encoding of sample identity.

Author information

1
Department of Neurobiology, Stanford University, Stanford, California 94305.

Abstract

Determining the genomic locations of transposable elements is a common experimental goal. When mapping large collections of transposon insertions, individualized amplification and sequencing is both time consuming and costly. We describe an approach in which large numbers of insertion lines can be simultaneously mapped in a single DNA sequencing reaction by using digital error-correcting codes to encode line identity in a unique set of barcoded pools.

KEYWORDS:

Drosophila melanogaster; mapping; next-generation sequencing; transposable elements

PMID:
24374352
PMCID:
PMC3948795
DOI:
10.1534/genetics.113.159483
[Indexed for MEDLINE]
Free PMC Article

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