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J Chromatogr A. 2014 Jan 24;1326:134-8. doi: 10.1016/j.chroma.2013.12.018. Epub 2013 Dec 15.

Penicillin G as a novel chiral selector in capillary electrophoresis.

Author information

1
Department of Chemistry, Yeungnam University, Gyeongsan 712-749, South Korea. Electronic address: drdixit.shuchi@gmail.com.
2
Department of Chemistry, Yeungnam University, Gyeongsan 712-749, South Korea. Electronic address: jhpark@ynu.ac.kr.

Abstract

The penicillin sub-class of β-lactam antibiotics has not been examined for its enantiodiscriminating abilities in capillary electrophoresis (CE) until date. The present work was therefore designed to evaluate penicillin G potassium salt (PenG) as an ion-pair chiral selector (CS) using CE for its several attributes, namely, high solubility in water and lower alcohols, structure allowing multiple interactions with analytes and cost-effectiveness. Systematic experiments were performed to investigate the effect of composition of background electrolyte, applied voltage and capillary temperature on chiral separation. Baseline resolutions of enantiomers of five basic chiral drugs (namely, darifenacin, citalopram, sertraline, propranolol and metoprolol) were attained using a background electrolyte composed of water:methanol (90:10, v/v) and consisting of 10.7 or 16.1mM CS at 20°C using an applied voltage of 5kV.

KEYWORDS:

CIT; CS; Capillary electrophoresis; Chiral selector; DAR; Enantioseparation; METO; MeCN; MeOH; PRO; PenG; Penicillin G; SERT; acetonitrile; chiral selector; citalopram hydrobromide; darifenacin; methanol; metoprolol; penicillin G; propranolol; sertraline hydrochloride

PMID:
24373536
DOI:
10.1016/j.chroma.2013.12.018
[Indexed for MEDLINE]

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