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Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2013 Dec;21(6):1552-6. doi: 10.7534/j.issn.1009-2137.2013.06.035.

[Long term in-vitro expansion reduces immune modulation function of placental chorionic villi mesenchymal stem cells].

[Article in Chinese]

Author information

1
State Key Laboratory of Experimental Hematology, Institute of Hematology & Hospital of Blood Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
2
State Key Laboratory of Experimental Hematology, Institute of Hematology & Hospital of Blood Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China; TEDA Life Science and Technology Research Center, Institute of Hematology, Chinese Academy of Medical Sciences, Tianjin 300457, China.
3
State Key Laboratory of Experimental Hematology, Institute of Hematology & Hospital of Blood Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China; TEDA Life Science and Technology Research Center, Institute of Hematology, Chinese Academy of Medical Sciences, Tianjin 300457, China. E-mail: hanzhongchao@hotmail.com.

Abstract

The main aim of this study was to investigate the biological activities and immune modulation changes of chorionic villi mesenchymal stem cells (CV-MSC) after long term culture. The morphology of the CV-MSC of passage 3 and passage 9 were observed by microscopy, and their phenotypes were detected by flow cytometry. CV-MSC of passage 3 and 9 were co-cultured with PHA-stimulated PBMNC, and IFN-γ concentration in culture medium was detected by ELISA. The mRNA expression of COX-2, HGF and HLA-G in CV-MSC were detected by real-time PCR. The results showed that after long term culture, the CV-MSC kept the MSC morphology and most of the phenotypes including CD31, CD34, CD44, CD45, CD62L, CD73, CD90, CD105, CD117, CD151, CD235a, CD271 and HLA-DR, while the CD49d was significantly up-regulated. Immune modulation ability of CV-MSC was reduced and the mRNA expression of COX-2 and HGF was down regulated after long term culture, but the expression of HLA-G did not found to be obvious change. It is concluded that the long term in vitro expansion changes the expression of CD49d and reduces immune modulation of CV-MSC.

PMID:
24370047
[Indexed for MEDLINE]

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