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J Gerontol A Biol Sci Med Sci. 2014 Dec;69(12):1437-47. doi: 10.1093/gerona/glt204. Epub 2013 Dec 24.

Human slow troponin T (TNNT1) pre-mRNA alternative splicing is an indicator of skeletal muscle response to resistance exercise in older adults.

Author information

1
Section on Gerontology and Geriatric Medicine, Department of Internal Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina.
2
Section on Gerontology and Geriatric Medicine, Department of Internal Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina. Present address: Division of Sports and Health, KyungSung University, Busan, South Korea.
3
Department of Physiology, Wayne State University School of Medicine.
4
Department of Health and Exercise Science, Wake Forest University, Winston-Salem, North Carolina.
5
Section on Gerontology and Geriatric Medicine, Department of Internal Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina. J Paul Sticht Center on Aging, Wake Forest School of Medicine, Winston-Salem, North Carolina.
6
Section on Gerontology and Geriatric Medicine, Department of Internal Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina. J Paul Sticht Center on Aging, Wake Forest School of Medicine, Winston-Salem, North Carolina. odelbono@wakehealth.edu.

Abstract

Slow skeletal muscle troponin T (TNNT1) pre-messenger RNA alternative splicing (AS) provides transcript diversity and increases the variety of proteins the gene encodes. Here, we identified three major TNNT1 splicing patterns (AS1-3), quantified their expression in the vastus lateralis muscle of older adults, and demonstrated that resistance training modifies their relative abundance; specifically, upregulating AS1 and downregulating AS2 and AS3. In addition, abundance of TNNT1 AS2 correlated negatively with single muscle fiber-specific force after resistance training, while abundance of AS1 correlated negatively with V max. We propose that TNNT1 AS1, AS2 and the AS1/AS2 ratio are potential quantitative biomarkers of skeletal muscle adaptation to resistance training in older adults, and that their profile reflects enhanced single fiber muscle force in the absence of significant increases in fiber cross-sectional area.

KEYWORDS:

Alternative splicing; Resistance exercise.; Skeletal muscle; Troponin T

PMID:
24368775
PMCID:
PMC4296115
DOI:
10.1093/gerona/glt204
[Indexed for MEDLINE]
Free PMC Article

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