Effects of gliotoxin on apoptosis-related proteins. Western blotting analysis of cyt c, Bcl-2 and Bax of the HeLa (A) and SW1353 (B) cells treated with 10, 30, 50, and 90 µM of gliotoxin, respectively. Western blotting analysis of cleavage forms of caspase-3, caspase-8 and caspase-9, and p53 of the HeLa (C) and SW1353 (D) cells treated with 10, 30, 50, and 90 µM of gliotoxin, respectively. Band intensity was normalized to β-actin expression by densitometry.