Primed Il17–GFP reporter mice infected with RFP Aspergillus showing representative corneas 24h post-infection (a), total RFP in infected corneas assessed by image analysis (b) (data points represent individual corneas), total NIMP- R14+ neutrophils (c), and percent IL-17/GFP expressing neutrophils in the corneal stroma (d). (e-j) IL-6+IL-23 – stimulated bone marrow neutrophils from Il17–GFP, Il17a-/-, Il17rc-/-, and Cybb-/- mice stimulated were injected intravenously into recipient Cd18-/- mice, which were then infected intrastromally with RFP Aspergillus. Representative corneas of Il17–GFP 24h post-infection (e), total RFP in infected corneas assessed by image analysis (f) (data points represent individual corneas), and total NIMP- R14+ neutrophils (g). Representative images of Aspergillus infected Cd18-/- corneas in mice given Il17a-/-, Il17rc-/-, or Cybb-/- neutrophils (h), total RFP Aspergillus (i), and total neutrophils (j). Experiments were repeated twice with similar results.