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Gynecol Oncol. 2014 Feb;132(2):383-8. doi: 10.1016/j.ygyno.2013.12.016. Epub 2013 Dec 18.

Application of the CellDetect® staining technique in diagnosis of human cervical cancer.

Author information

1
Department of Pathology, Affiliated Tumor Hospital, Nantong University, Nantong, China.
2
Department of Pathological Anatomy, Nantong University, Nantong, China.
3
Biomics Biotechnologies Co., Ltd, Nantong, China.
4
Department of Pathology, Affiliated Tumor Hospital, Nantong University, Nantong, China; Department of Pathological Anatomy, Nantong University, Nantong, China. Electronic address: bl1@ntu.edu.cn.

Abstract

BACKGROUND:

CellDetect® staining technique is a newly invented technique for cancer diagnosis. It easily distinguishes between normal and neoplastic cells including pre-cancer and squamous cell carcinoma (SCC) cells, based on staining color and morphology. In this study, application of CellDetect® staining technique was assessed in diagnosis of human cervical cancer as compared with hematoxylin and eosin (H&E) staining in conventional slides and Thinprep cytologic test (TCT) smears.

METHODS:

The conventional slides and TCT smears of 600 patients were stained and observed while comparing with H&E staining to assess sensitivity and specificity of CellDetect® staining technique in diagnosis of cervical cancer. Conventional smear slides (440 cases) were fixed in 95% ethanol or with CYTOFIX® Spray. TCT smears (160 cases) were processed based on manual. The paraffin sections from cervical intraepithelium neoplasia (CIN) 2-3 and SCC cases were prepared by biopsy.

RESULTS:

CellDetect® staining exhibited well cell morphology, simultaneously, showed dual color discrimination, the stain targeted cytoplasm in normal cells in green and dysplastic cells or neoplastic cells in purple/red. Both cervical cell smears or both fixation methods in conventional slides did not affect CellDetect® staining diagnosis, especially in tissue biopsies CellDetect® staining exhibited well epithelium layers to benefit the diagnosis of CIN grade. The sensitivity and specificity of CellDetect® staining technology in diagnosing CIN and SCC were 94.34% and 88.73%, respectively.

CONCLUSIONS:

CellDetect® staining technique provided a dual color discrimination and morphological analysis. It has the potential to become one of the most effective methods for cervical screening and early diagnosis.

KEYWORDS:

CellDetect® stain; Cervical exfoliated cells; Cervical neoplasia

PMID:
24361533
DOI:
10.1016/j.ygyno.2013.12.016
[Indexed for MEDLINE]
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