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Cell Rep. 2013 Dec 26;5(6):1749-62. doi: 10.1016/j.celrep.2013.11.023. Epub 2013 Dec 19.

Interactome of two diverse RNA granules links mRNA localization to translational repression in neurons.

Author information

1
Department of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, Austria.
2
Department of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, Austria; Max-Planck-Institute for Developmental Biology, 72076 Tübingen, Germany.
3
CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, 1090 Vienna, Austria.
4
Department of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, Austria; Department for Anatomy & Cell Biology, Ludwig-Maximilians-University, 80336 Munich, Germany.
5
Department of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, Austria; Max-Planck-Institute for Developmental Biology, 72076 Tübingen, Germany; Department for Anatomy & Cell Biology, Ludwig-Maximilians-University, 80336 Munich, Germany.
6
Department of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, Austria; Max-Planck-Institute for Developmental Biology, 72076 Tübingen, Germany; Department for Anatomy & Cell Biology, Ludwig-Maximilians-University, 80336 Munich, Germany. Electronic address: michael.kiebler@med.uni-muenchen.de.

Abstract

Transport of RNAs to dendrites occurs in neuronal RNA granules, which allows local synthesis of specific proteins at active synapses on demand, thereby contributing to learning and memory. To gain insight into the machinery controlling dendritic mRNA localization and translation, we established a stringent protocol to biochemically purify RNA granules from rat brain. Here, we identified a specific set of interactors for two RNA-binding proteins that are known components of neuronal RNA granules, Barentsz and Staufen2. First, neuronal RNA granules are much more heterogeneous than previously anticipated, sharing only a third of the identified proteins. Second, dendritically localized mRNAs, e.g., Arc and CaMKIIα, associate selectively with distinct RNA granules. Third, our work identifies a series of factors with known roles in RNA localization, translational control, and RNA quality control that are likely to keep localized transcripts in a translationally repressed state, often in distinct types of RNPs.

PMID:
24360960
DOI:
10.1016/j.celrep.2013.11.023
[Indexed for MEDLINE]
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