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Int J Biol Macromol. 2014 Mar;64:313-8. doi: 10.1016/j.ijbiomac.2013.12.019. Epub 2013 Dec 18.

Isolation and characterization of peptidoglycan recognition protein 1 from antler base of sika deer (Cervus nippon).

Author information

1
College of Biological and Agricultural Engineering, Jilin University, Changchun 130022, PR China.
2
College of Biological and Agricultural Engineering, Jilin University, Changchun 130022, PR China. Electronic address: jlu_jw@163.com.

Abstract

Peptidoglycan recognition proteins (PGRPs) are secreted innate immunity pattern recognition molecules. In this study, a new peptidoglycan recognition protein 1 named cnPGRP1 was isolated from an antler base of sika deer Cervus nippon. The antler base antimicrobial proteins (AAP) were subjected to consecutive chromatographic methods connected to Sephadex G-25 gel filtration column (CM) anion-exchange column, and RP-HPLC. The molecular weight of cnPGRP1 was 17.2 kDa under SDS-PAGE, and peptide mass fingerprint analysis by MALDI-TOF-MS as peptidoglycan recognition protein 1 matched to Dasypus novemcinctus. The matched amino acids sequences were RLYEIIQKWPHYRA. Both Gram-positive and Gram-negative bacteria can be killed by cnPGRP1 in the 50-250 μg/mL range through in vitro. Furthermore, cnPGRP1 has been found to bind Gram-positive bacteria, Gram-negative bacteria, and even fungus.

KEYWORDS:

Antimicrobial activity; Antler base; MALDI-TOF-MS; Peptidoglycan recognition protein 1

PMID:
24360898
DOI:
10.1016/j.ijbiomac.2013.12.019
[Indexed for MEDLINE]

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