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Mol Genet Metab. 2014 Feb;111(2):205-8. doi: 10.1016/j.ymgme.2013.11.011. Epub 2013 Dec 1.

In vitro effect of genistein on DNA damage in leukocytes from mucopolysaccharidosis IVA patients.

Author information

1
Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Ipiranga, 2752, 1º andar, CEP: 90610-000 Porto Alegre, RS, Brazil; Serviço de Genética Médica, Hospital de Clínicas de Porto Alegre (HCPA), Ramiro Barcelos 2350, Porto Alegre, RS 90035-903, Brazil. Electronic address: gnegretto@gmail.com.
2
Serviço de Genética Médica, Hospital de Clínicas de Porto Alegre (HCPA), Ramiro Barcelos 2350, Porto Alegre, RS 90035-903, Brazil.
3
Faculdade de Farmácia, UFRGS, Laboratório de Toxicologia, Endereço: Avenida Ipiranga, 2752, sala 605, CEP: 90610-000, Brazil.
4
Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Ipiranga, 2752, 1º andar, CEP: 90610-000 Porto Alegre, RS, Brazil; Serviço de Genética Médica, Hospital de Clínicas de Porto Alegre (HCPA), Ramiro Barcelos 2350, Porto Alegre, RS 90035-903, Brazil. Electronic address: crvargas@hcpa.ufrgs.br.

Abstract

Mucopolysaccharidosis IVA is a lysosomal storage disorder leading to an increase in glycosaminoglycans storage. Genistein is an isoflavone capable to inhibit glycosaminoglycans production. The objective of this study was to analyze the in vitro effect of different concentrations of genistein on DNA injury in mucopolysaccharidosis IVA patients. The lower concentration tested (10 μM) showed a significant increase on DNA injury in vitro, although higher concentrations (30 μM and 50 μM) showed higher DNA damage.

KEYWORDS:

Comet assay; DNA damage; Genistein; Genotoxicity; Mucopolysaccharidosis IVA

PMID:
24359664
DOI:
10.1016/j.ymgme.2013.11.011
[Indexed for MEDLINE]
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