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Chembiochem. 2014 Jan 3;15(1):37-41. doi: 10.1002/cbic.201300608. Epub 2013 Dec 16.

E1-catalyzed ubiquitin C-terminal amidation for the facile synthesis of deubiquitinase substrates.

Author information

1
Department of Chemistry, Texas A&M University, College Station, TX 77843 (USA).

Abstract

Will Ub my partner? The ubiquitin (Ub)-activating enzyme (E1) was used to catalyze an amidation reaction to functionalize the C terminus of Ub with unique functional groups, such as thiol, azide, alkyne, and alkene groups, with high efficiency and yield (>90 %). These groups were then applied for the facile synthesis of fluorophore-conjugated ubiquitin and specifically conjugated diubiquitin substrates for deubiquitinases.

KEYWORDS:

deubiquitinases; diubiquitin; protein engineering; synthetic biology; ubiquitin functionalization

PMID:
24357003
DOI:
10.1002/cbic.201300608
[Indexed for MEDLINE]

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