Format

Send to

Choose Destination
See comment in PubMed Commons below
RNA Biol. 2013 Dec;10(12):1807-14. doi: 10.4161/rna.27239. Epub 2013 Nov 22.

MiR-193b and miR-365-1 are not required for the development and function of brown fat in the mouse.

Author information

1
Laboratory of Genetics and Physiology; NIDDK, NIH; Bethesda, MD USA.
2
Mouse Metabolic Core; NIDDK, NIH; Bethesda, MD USA.
3
Laboratory of Genetics and Physiology; NIDDK, NIH; Bethesda, MD USA; Georg-Speyer-Haus, Institute for Tumor Biology and Experimental Therapy and LOEWE Center for Cell and Gene Therapy Frankfurt and Department of Hematology/Oncology; Goethe University; Frankfurt am Main, Germany.
4
Laboratory of Metabolism, Center for Cancer Research, NCI; NIH; Bethesda, MD USA.

Abstract

Generating heat and maintaining body temperature is the primary function of brown adipose tissue (BAT). Previous studies have implicated microRNAs, including miR-193b and miR-365-1, in BAT differentiation. We used mouse genetics to further understand the specific contributions of these two miRs. BAT function in mice with an inactivated miR-193b-365-1 locus, as determined by their response to the selective β3 adrenergic receptor agonist CL316.243 and their tolerance to cold exposure, was normal and expression of genes associated with functional BAT, including Prdm16 and Ucp1, was unaffected. In addition, genome-wide expression profiles of miRNAs and mRNAs in BAT in the presence and absence of miR-193b-365-1 were determined. In summary, these data demonstrate, in contrast to earlier work, that the development, differentiation, and function of BAT do not require the presence of miR-193b and miR-365-1.

PMID:
24356587
PMCID:
PMC3917983
DOI:
10.4161/rna.27239
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Taylor & Francis Icon for PubMed Central
    Loading ...
    Support Center