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Vet J. 2014 Feb;199(2):306-7. doi: 10.1016/j.tvjl.2013.11.008. Epub 2013 Nov 19.

Allele frequency of hereditary equine regional dermal asthenia in American Quarter horses in Brazil determined by quantitative real-time PCR with high resolution melting analysis.

Author information

1
School of Veterinary Medicine and Animal Science, Universidade Estadual Paulista, Department of Veterinary Clinical Science, Botucatu, São Paulo, Brazil.
2
College of Veterinary Medicine, Cornell University, Department of Molecular Medicine, Ithaca, NY, USA.
3
School of Veterinary Medicine and Animal Science, Universidade Estadual Paulista, Department of Veterinary Clinical Science, Botucatu, São Paulo, Brazil. Electronic address: asborges@fmvz.unesp.br.

Abstract

Hereditary equine regional dermal asthenia (HERDA) is a genetic disorder that occurs in the American Quarter horse (AQH) and is caused by a c.115G>A missense mutation in the peptidylprolyl isomerase B (PPIB) gene. Using a quantitative real-time PCR high resolution melting analysis genotyping assay for the PPIB mutation, the estimated HERDA allele and carrier frequencies in a sample of Brazilian AQHs were 2.9% and 5.8%, respectively.

KEYWORDS:

Cyclophilin B; Genotyping; Hereditary equine regional dermal asthenia; High resolution melting analysis; Quantitative real-time PCR

PMID:
24355815
DOI:
10.1016/j.tvjl.2013.11.008
[Indexed for MEDLINE]

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