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Exp Cell Res. 2014 Feb 15;321(2):99-108. doi: 10.1016/j.yexcr.2013.12.010. Epub 2013 Dec 16.

Ex vivo cultures of glioblastoma in three-dimensional hydrogel maintain the original tumor growth behavior and are suitable for preclinical drug and radiation sensitivity screening.

Author information

1
Aix Marseille Université, Faculté de Médecine de la Timone, 27 boulevard Jean Moulin, 13284 Marseille, France; CRO2, UMR 911, Faculté de Médecine de la Timone, 27 boulevard Jean Moulin, 13284 Marseille Cedex, France; INSERM, U911, 13005 Marseille, France. Electronic address: carine.jiguet-jiglaire@univ-amu.fr.
2
Aix Marseille Université, Faculté de Médecine de la Timone, 27 boulevard Jean Moulin, 13284 Marseille, France; CRO2, UMR 911, Faculté de Médecine de la Timone, 27 boulevard Jean Moulin, 13284 Marseille Cedex, France; INSERM, U911, 13005 Marseille, France.
3
Aix Marseille Université, Faculté de Médecine de la Timone, 27 boulevard Jean Moulin, 13284 Marseille, France; CRO2, UMR 911, Faculté de Médecine de la Timone, 27 boulevard Jean Moulin, 13284 Marseille Cedex, France; INSERM, U911, 13005 Marseille, France; APHM, Timone Hospital, Department of Neurosurgery, 13005 Marseille, France; Timone Hospital, 264 Rue Saint Pierre, 13385 Marseille Cedex 5, France.
4
Aix Marseille Université, Faculté de Médecine de la Timone, 27 boulevard Jean Moulin, 13284 Marseille, France; CRO2, UMR 911, Faculté de Médecine de la Timone, 27 boulevard Jean Moulin, 13284 Marseille Cedex, France; INSERM, U911, 13005 Marseille, France; Timone Hospital, 264 Rue Saint Pierre, 13385 Marseille Cedex 5, France; APHM, Timone Hospital, Department of Radiotherapy, 13005 Marseille, France.
5
Aix Marseille Université, Faculté de Médecine de la Timone, 27 boulevard Jean Moulin, 13284 Marseille, France; CRO2, UMR 911, Faculté de Médecine de la Timone, 27 boulevard Jean Moulin, 13284 Marseille Cedex, France; INSERM, U911, 13005 Marseille, France; Timone Hospital, 264 Rue Saint Pierre, 13385 Marseille Cedex 5, France; APHM, Timone Hospital, Department of Neurooncology, 13005 Marseille, France.
6
Aix Marseille Université, Faculté de Médecine de la Timone, 27 boulevard Jean Moulin, 13284 Marseille, France; CRO2, UMR 911, Faculté de Médecine de la Timone, 27 boulevard Jean Moulin, 13284 Marseille Cedex, France; INSERM, U911, 13005 Marseille, France; Timone Hospital, 264 Rue Saint Pierre, 13385 Marseille Cedex 5, France; APHM, Timone Hospital, Department of Pathology, 13005 Marseille, France.

Abstract

Identification of new drugs and predicting drug response are major challenges in oncology, especially for brain tumors, because total surgical resection is difficult and radiation therapy or chemotherapy is often ineffective. With the aim of developing a culture system close to in vivo conditions for testing new drugs, we characterized an ex vivo three-dimensional culture system based on a hyaluronic acid-rich hydrogel and compared it with classical two-dimensional culture conditions. U87-MG glioblastoma cells and seven primary cell cultures of human glioblastomas were subjected to radiation therapy and chemotherapy drugs. It appears that 3D hydrogel preserves the original cancer growth behavior and enables assessment of the sensitivity of malignant gliomas to radiation and drugs with regard to inter-tumoral heterogeneity of therapeutic response. It could be used for preclinical assessment of new therapies.

KEYWORDS:

3D environment; Glioblastoma; Preclinical tool; Sensitivity screening

PMID:
24355810
DOI:
10.1016/j.yexcr.2013.12.010
[Indexed for MEDLINE]

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