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Environ Toxicol Pharmacol. 2014 Jan;37(1):158-65. doi: 10.1016/j.etap.2013.11.021. Epub 2013 Dec 1.

Aspartame-induced apoptosis in PC12 cells.

Author information

1
Environmental Adaptation Science, Division of Environmental Science Development, Graduate School of Environmental Science, Hokkaido University, Sapporo 060-0810, Japan.
2
Laboratory of Environmental Health Sciences, Faculty of Health Sciences, Hokkaido University, Sapporo 060-0812, Japan.
3
Environmental Adaptation Science, Division of Environmental Science Development, Graduate School of Environmental Science, Hokkaido University, Sapporo 060-0810, Japan; Group of Environmental Adaptation Science, Faculty of Environmental Earth Science, Hokkaido University, Sapporo 060-0810, Japan. Electronic address: kura@ees.hokudai.ac.jp.

Abstract

Aspartame is an artificial sweetner added to many low-calorie foods. The safety of aspartame remains controversial even though there are many studies on its risks. In this study, to understand the physiological effects of trace amounts of artificial sweetners on cells, the effects of aspartame on apoptosis were investigated using a PC12 cell system. In addition, the mechanism of apoptosis induced by aspartame in PC12 cells and effects on apoptotic factors such as cytochrome c, apoptosis-inducing factor, and caspase family proteins were studied by Western blotting and RT-PCR. Aspartame-induced apoptosis in PC12 cells in a dose-dependent manner. In addition, aspartame exposure increased the expressions of caspases 8 and 9, and cytochrome c. These results indicate that aspartame induces apoptosis mainly via mitochondrial pathway involved in apoptosis due to oxigen toxicity.

KEYWORDS:

AIF; Apoptosis; Aspartame; Caspases; Cytochrome c; PC12

PMID:
24355796
DOI:
10.1016/j.etap.2013.11.021
[Indexed for MEDLINE]

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