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J Biosci Bioeng. 2014 Jun;117(6):676-9. doi: 10.1016/j.jbiosc.2013.11.010. Epub 2013 Dec 17.

Galactosylation of human erythropoietin produced by chimeric chickens expressing galactosyltransferase.

Author information

1
Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
2
Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan. Electronic address: kaneoka@nubio.nagoya-u.ac.jp.

Abstract

Human erythropoietin produced in the egg white of chimeric chicken contains N-glycan with lower amounts of terminal galactose and sialic acid; therefore, the chicken galactosyltransferase gene was introduced together with the human erythropoietin gene by a retroviral vector. We found that erythropoietin accumulated in the egg white was partially galactosylated.

KEYWORDS:

Erythropoietin; Galactose; Galactosyltransferase; N-Glycan; Transgenic chicken

PMID:
24355574
DOI:
10.1016/j.jbiosc.2013.11.010
[Indexed for MEDLINE]

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