Format

Send to

Choose Destination
Clin Exp Otorhinolaryngol. 2013 Dec;6(4):237-42. doi: 10.3342/ceo.2013.6.4.237. Epub 2013 Nov 29.

Effect of Epigallocatechin-3-Gallate on PMA-Induced MUC5B Expression in Human Airway Epithelial Cells.

Author information

1
Department of Physiology, Yeungnam University College of Medicine, Daegu, Korea.
2
Department of Otorhinolaryngology-Head and Neck Surgery, Yeungnam University College of Medicine, Daegu, Korea.
3
Department of Otorhinolaryngology-Head and Neck Surgery, Yeungnam University College of Medicine, Daegu, Korea. ; Regional Center for Respiratory Diseases, Yeungnam University Medical Center, Daegu, Korea.

Abstract

OBJECTIVES:

Among the inflammatory mediators, phorbol 12-myristate 13-acetate (PMA) is associated with the regulation of MUC5B expression in the airway epithelial cells. Epigallocatechin-3-gallate (EGCG) is the major component of green tea extract. The biological activity of EGCG includes reduction of cholesterol and antioxidant activity, as well as anti-inflammatory effect. However, the precise action mechanism of anti-inflammatory effect of EGCG in the airway epithelial cells has not been fully defined. This study investigates the effect and the brief signaling pathway of EGCG on PMA-induced MUC5B expression in the airway epithelial cells.

METHODS:

In NCI-H292 airway epithelial cells, the effect and signaling pathway of EGCG on MUC5B expression were investigated using real-time polymerase chain reaction analysis, enzyme immunoassay, immunohistochemical analysis, gelatin zymography assay, and immunoblot analysis.

RESULTS:

In NCI-H292 airway epithelial cells, PMA induced MUC5B expression, phosphorylation of p38 mitogen-activated protein kinase (MAPK), and matrix metalloproteinase-9 (MMP-9) expression and protein activity. EGCG significantly decreased PMA-induced MUC5B expression, phosphorylation of p38 MAPK, and MMP-9 expression and protein activity. SB203580 (p38 MAPK inhibitor) significantly decreased PMA-induced MMP-9 expression. In addition, SB203580 and MMP-9 I (MMP-9 inhibitor) significantly decreased PMA-induced MUC5B expression.

CONCLUSION:

These results suggest that EGCG down-regulates PMA-induced MUC5B expression through the p38 MAPK dependent MMP-9 signaling pathway in human airway epithelial cells.

KEYWORDS:

Airway epithelial cell; Epigallocatechin-3-gallate; MUC5B; Matrix metalloproteinase-9; p38

Supplemental Content

Full text links

Icon for Korean Society of Otorhinolaryngology-Head and Neck Surgery Icon for PubMed Central
Loading ...
Support Center