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J Neurophysiol. 2014 Mar;111(5):1132-49. doi: 10.1152/jn.00785.2013. Epub 2013 Dec 18.

Large-scale, high-density (up to 512 channels) recording of local circuits in behaving animals.

Author information

1
New York University Neuroscience Institute, School of Medicine, New York University, New York, New York;

Abstract

Monitoring representative fractions of neurons from multiple brain circuits in behaving animals is necessary for understanding neuronal computation. Here, we describe a system that allows high-channel-count recordings from a small volume of neuronal tissue using a lightweight signal multiplexing headstage that permits free behavior of small rodents. The system integrates multishank, high-density recording silicon probes, ultraflexible interconnects, and a miniaturized microdrive. These improvements allowed for simultaneous recordings of local field potentials and unit activity from hundreds of sites without confining free movements of the animal. The advantages of large-scale recordings are illustrated by determining the electroanatomic boundaries of layers and regions in the hippocampus and neocortex and constructing a circuit diagram of functional connections among neurons in real anatomic space. These methods will allow the investigation of circuit operations and behavior-dependent interregional interactions for testing hypotheses of neural networks and brain function.

KEYWORDS:

behaving rats and mice; local field potential; monosynaptic connections; unit firing

PMID:
24353300
PMCID:
PMC3949233
DOI:
10.1152/jn.00785.2013
[Indexed for MEDLINE]
Free PMC Article
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