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Nat Commun. 2013;4:2911. doi: 10.1038/ncomms3911.

Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography.

Author information

1
Department of Chemistry and Molecular Biology, University of Gothenburg, 405 30 Gothenburg, Sweden.
2
Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
3
1] Max-Planck-Institut für Medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany [2] Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany.
4
Linac Coherent Light Source, LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, 94025 California, USA.
5
PULSE Institute, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, 94025 California, USA.
6
Department of Chemistry-Ångström Laboratory, Uppsala University, Box 523, 75120 Uppsala, Sweden.
7
Department of Physics, Arizona State University, Tempe, 85287 Arizona, USA.
8
Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, 94550 California, USA.
9
Department of Chemistry and Biochemistry, Arizona State University, Tempe, 85287-1604 Arizona, USA.
10
1] Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany [2] Department of Physics, University of Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany.
11
Joint Laboratory for Structural Biology of Infection and Inflammation, Institute of Biochemistry and Molecular Biology, University of Hamburg and Institute of Biochemistry, University of Lübeck at DESY, 22607 Hamburg, Germany.

Abstract

Serial femtosecond crystallography is an X-ray free-electron-laser-based method with considerable potential to have an impact on challenging problems in structural biology. Here we present X-ray diffraction data recorded from microcrystals of the Blastochloris viridis photosynthetic reaction centre to 2.8 Å resolution and determine its serial femtosecond crystallography structure to 3.5 Å resolution. Although every microcrystal is exposed to a dose of 33 MGy, no signs of X-ray-induced radiation damage are visible in this integral membrane protein structure.

PMID:
24352554
PMCID:
PMC3905732
DOI:
10.1038/ncomms3911
[Indexed for MEDLINE]
Free PMC Article
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