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Zhonghua Yi Xue Za Zhi. 2013 Aug 27;93(32):2549-52.

[Loss of expression and mechanism of early growth response gene-3 in human oral squamous carcinoma].

[Article in Chinese]

Author information

1
Department of Prothodontics, Beijing Stomatological Hospital, Capital Medical University,Beijing 100050, China.
2
Email: zhenting_zhang@126.com.

Abstract

OBJECTIVE:

To evaluate the expression of early growth response gene-3 (EGR3) in human oral squamous carcinoma tissue, non-adjacent tissue and oral carcinoma cell lines and detect the effects of EGR3 expression on the biological behaviors of oral squamous carcinoma cell lines.

METHODS:

From October 2008 to December 2009, the expression of EGR3 was detected in 20 human oral squamous carcinoma tissue, non-tumor adjacent tissue and 2 oral carcinoma cell lines. The total-length plasmid of EGR3 was constructed and transfected into oral squamous carcinoma line Tca-8113 to observe the proliferation and apoptosis.

RESULTS:

There was a high-level expression of EGR3 mRNA in normal oral squamous epithelial and non-tumor adjacent tissues (2.108 ± 0.996 and 1.721 ± 1.196). And a low-level expression or a loss of EGR3 mRNA in human oral squamous carcinoma tissue and oral carcinoma cell lines (0.007 ± 0.005 and 0.007 ± 0.001) (both P < 0.05). Tca-8113 transfected by EGR3 plasmid had a high-level expression of EGR3 mRNA capable of inhibiting the proliferation of Tca-8113 and promoting its apoptosis dramatically. The stimulating index in overexpression of EGR3 was lower than control group (0.35 ± 0.11 vs 0.82 ± 0.21, P < 0.05). After overexpression of EGR3 in Tca-8113, the frequency of middle-Late stage of apoptosis (Annexin-V/PI double positive) was higher than the frequency of vector group (23.12% ± 6.65% vs 5.96% ± 0.98%, P < 0.05).

CONCLUSION:

A low-level expression or a loss of EGR3 in human oral squamous carcinoma and an over-expression of EGR3 in human oral squamous carcinoma may inhibit the proliferation and promote apoptosis of oral squamous carcinoma.

PMID:
24351594
[Indexed for MEDLINE]
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