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PLoS One. 2013 Dec 4;8(12):e80931. doi: 10.1371/journal.pone.0080931. eCollection 2013.

Ubiquitin-specific peptidase 5, a target molecule of vialinin A, is a key molecule of TNF-α production in RBL-2H3 cells.

Author information

1
Department of Nutritional Science, Faculty of Applied Bio-science, Tokyo University of Agriculture, Tokyo, Japan.
2
Department of Bioscience, Faculty of Applied Bio-science, Tokyo University of Agriculture, Tokyo, Japan.
3
Department of Fermentation Science, Faculty of Applied Bio-science, Tokyo University of Agriculture, Tokyo, Japan.
4
RIKEN (The Institute of Physical and Chemical Research), Wako, Saitama, Japan.

Abstract

Tumor necrosis factor alpha (TNF-α), a central mediator of the inflammatory response, is released from basophilic cells and other cells in response to a variety of proinflammatory stimuli. Vialinin A is a potent inhibitor of TNF-α production and is released from RBL-2H3 cells. Ubiquitin-specific peptidase 5 (USP5), a deubiquitinating enzyme, was identified as a target molecule of vialinin A and its enzymatic activity was inhibited by vialinin A. Here we report production of TNF-α is decreased in USP5 siRNA-knockdown RBL-2H3 cells, compared with control cells. The finding of the present study strongly suggests that USP5 is one of the essential molecules for the production of TNF-α in RBL-2H3.

PMID:
24349023
PMCID:
PMC3857809
DOI:
10.1371/journal.pone.0080931
[Indexed for MEDLINE]
Free PMC Article
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