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Magn Reson Med. 2014 Jan;71(1):19-25. doi: 10.1002/mrm.25003. Epub 2013 Nov 11.

Dynamic hyperpolarized carbon-13 MR metabolic imaging of nonhuman primate brain.

Author information

1
Surbeck Laboratory of Advanced Imaging, Department of Radiology and Biomedical Imaging, University of California, San Francisco, California, USA.

Abstract

PURPOSE:

To investigate hyperpolarized (13) C metabolic imaging methods in the primate brain that can be translated into future clinical trials for patients with brain cancer.

METHODS:

(13) C coils and pulse sequences designed for use in humans were tested in phantoms. Dynamic (13) C data were obtained from a healthy cynomolgus monkey brain using the optimized (13) C coils and pulse sequences. The metabolite kinetics were estimated from two-dimensional localized (13) C dynamic imaging data from the nonhuman primate brain.

RESULTS:

Pyruvate and lactate signal were observed in both the brain and the surrounding tissues with the maximum signal-to-noise ratio of 218 and 29 for pyruvate and lactate, respectively. Apparent rate constants for the conversion of pyruvate to lactate and the ratio of lactate to pyruvate showed a difference between brain and surrounding tissues.

CONCLUSION:

The feasibility of using hyperpolarized [1-(13) C]-pyruvate for assessing in vivo metabolism in a healthy nonhuman primate brain was demonstrated using a hyperpolarized (13) C imaging experimental setup designed for studying patients with brain tumors. The kinetics of the metabolite conversion suggests that this approach may be useful in future studies of human neuropathology.

KEYWORDS:

Hyperpolarized carbon-13 magnetic resonance spectroscopic imaging; dynamic nuclear polarization; primate brain; pyruvate

PMID:
24346964
PMCID:
PMC4041734
DOI:
10.1002/mrm.25003
[Indexed for MEDLINE]
Free PMC Article

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