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J Am Vet Med Assoc. 2014 Jan 1;244(1):63-7. doi: 10.2460/javma.244.1.63.

Refractometric total protein concentrations in icteric serum from dogs.

Author information

1
Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506.

Abstract

OBJECTIVE:

To determine whether high serum bilirubin concentrations interfere with the measurement of serum total protein concentration by refractometry and to assess potential biases among refractometer measurements.

DESIGN:

Evaluation study.

SAMPLE:

Sera from 2 healthy Greyhounds.

PROCEDURES:

Bilirubin was dissolved in 0.1M NaOH, and the resulting solution was mixed with sera from 2 dogs from which food had been withheld to achieve various bilirubin concentrations up to 40 mg/dL. Refractometric total protein concentrations were estimated with 3 clinical refractometers. A biochemical analyzer was used to measure biuret assay-based total protein and bilirubin concentrations with spectrophotometric assays.

RESULTS:

No interference with refractometric measurement of total protein concentrations was detected with bilirubin concentrations up to 41.5 mg/dL. Biases in refractometric total protein concentrations were detected and were related to the conversion of refractive index values to total protein concentrations.

CONCLUSIONS AND CLINICAL RELEVANCE:

Hyperbilirubinemia did not interfere with the refractometric estimation of serum total protein concentration. The agreement among total protein concentrations estimated by 3 refractometers was dependent on the method of conversion of refractive index to total protein concentration and was independent of hyperbilirubinemia.

PMID:
24344854
DOI:
10.2460/javma.244.1.63
[Indexed for MEDLINE]

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