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Biotechniques. 2013 Dec;55(6):314-6. doi: 10.2144/000114116.

A method for high-throughput PCR-based genotyping of larval zebrafish tail biopsies.

Author information

1
Department of Cardiovascular Science, University of Sheffield, Medical School, Sheffield, United Kingdom.
2
Department of Biomedical Science/ MRC Centre for Developmental and Biomedical Genetics, University of Sheffield, Sheffield, United Kingdom.

Abstract

Here we describe a method for high-throughput genotyping of live larval zebrafish as early as 72 h post-fertilization (hpf). Importantly, this technique allows rapid and cost-effective PCR-based genotyping from very small fin biopsies, which regenerate as the embryo develops, thereby allowing researchers to select embryos with desired genotypes to be raised to adulthood.

KEYWORDS:

PCR; embryo; fin clip; genotyping; zebrafish

PMID:
24344681
DOI:
10.2144/000114116
[Indexed for MEDLINE]
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