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Am J Clin Pathol. 2014 Jan;141(1):52-61. doi: 10.1309/AJCPBLFBNAP6N2UN.

Increased concentrations of apo A-I and apo A-II fragments in the serum of patients with hepatocellular carcinoma by magnetic beads-assisted MALDI-TOF mass spectrometry.

Author information

1
Dept of Molecular Diagnosis, Graduate School of Medicine, Division of Laboratory Medicine, Clinical Genetic and Proteomics, Chiba University Hospital, 1-8-1 Inohana, Chiba City, Chiba 260-8670, Japan; fnomura@faculty.chiba-u.jp.

Abstract

OBJECTIVES:

Recent advances in sophisticated technologies in proteomics should provide promising ways to discover novel markers for hepatocellular carcinoma (HCC) in the early diagnosis.

METHODS:

Serum peptide and protein profiling was conducted by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Profiling was carried out in a training set of 16 patients with HCC and a testing set of 15 patients with cirrhosis without HCC. All the patients were hepatitis C virus positive. Candidate peaks were processed to partial purification, followed by protein identification by amino acid sequence analysis. Immunoprecipitation was conducted to confirm the protein identity.

RESULTS:

Partial purification and protein identification revealed that one peak that was up-regulated in HCC sera both in the training and the testing sets was a fragment of apolipoprotein A-I (apo A-I). Immunoprecipitation confirmed this result.

CONCLUSIONS:

MALDI-TOF MS analysis revealed that apo A-I is a potential novel serum marker of HCC. Combination of these pretreatments and the current magnet bead-assisted MALDI-TOF MS will further enhance the efficiency of biomarker discovery for HCC.

KEYWORDS:

Apolipoprotein A-I; Apolipoprotein A-II; Hepatocellular carcinoma; MALDI-TOF MS

PMID:
24343737
DOI:
10.1309/AJCPBLFBNAP6N2UN
[Indexed for MEDLINE]
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