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Biomaterials. 2014 Feb;35(7):2089-2096. doi: 10.1016/j.biomaterials.2013.11.040. Epub 2013 Dec 14.

Optical imaging of fibrin deposition to elucidate participation of mast cells in foreign body responses.

Author information

1
Department of Bioengineering, University of Texas at Arlington, Arlington, TX 76019, USA.
2
Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
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Contributed equally

Abstract

Mast cell activation has been shown to be an initiator and a key determinant of foreign body reactions. However, there is no non-invasive method that can quantify the degree of implant-associated mast cell activation. Taking advantage of the fact that fibrin deposition is a hallmark of mast cell activation around biomaterial implants, a near infrared probe was fabricated to have high affinity to fibrin. Subsequent in vitro testing confirmed that this probe has high affinity to fibrin. Using a subcutaneous particle implantation model, we found significant accumulation of fibrin-affinity probes at the implant sites as early as 15 min following particle implantation. The accumulation of fibrin-affinity probes at the implantation sites could also be substantially reduced if anti-coagulant - heparin was administered at the implant sites. Further studies have shown that subcutaneous administration of mast cell activator - compound 48/80 - prompted the accumulation of fibrin-affinity probes. However, implant-associated fibrin-affinity probe accumulation was substantially reduced in mice with mast cell deficiency. The results show that our fibrin-affinity probes may serve as a powerful tool to monitor and measure the extent of biomaterial-mediated fibrin deposition and mast cell activation in vivo.

KEYWORDS:

Biocompatibility; Biomaterial; Fibrin; Inflammation; In vivo imaging; Near infrared fluorescence

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