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Biochem Biophys Res Commun. 2014 Jan 10;443(2):768-74. doi: 10.1016/j.bbrc.2013.12.040. Epub 2013 Dec 14.

Coordinated transcriptional regulation of isopentenyl diphosphate biosynthetic pathway enzymes in plastids by phytochrome-interacting factor 5.

Author information

1
Graduate School of Engineering, Tohoku University, Sendai, Miyagi 980-8579, Japan.
2
Graduate School of Engineering, Tohoku University, Sendai, Miyagi 980-8579, Japan. Electronic address: takahasi@seika.che.tohoku.ac.jp.
3
Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan.
4
Institute of Multidisciplinary Research for Advanced Material, Tohoku University, Sendai, Miyagi 980-8577, Japan.

Abstract

All isoprenoids are derived from a common C5 unit, isopentenyl diphosphate (IPP). In plants, IPP is synthesized via two distinct pathways; the cytosolic mevalonate pathway and the plastidial non-mevalonate (MEP) pathway. In this study, we used a co-expression analysis to identify transcription factors that coordinately regulate the expression of multiple genes encoding enzymes in the IPP biosynthetic pathway. Some candidates showed especially strong correlations with multiple genes encoding MEP-pathway enzymes. We report here that phytochrome-interacting factor 5 (PIF5), a basic-helix-loop-helix type transcription factor, functions as a positive regulator of the MEP pathway. Its overexpression in T87 suspension cultured cells resulted in increased accumulation of chlorophylls and carotenoids. Detailed analyses of carotenoids by HPLC indicated that some carotenoid biosynthetic pathways were concomitantly up-regulated, possibly as a result of enhanced IPP metabolic flow. Our results also revealed other PIF family proteins that play different roles from that of PIF5 in IPP metabolism.

KEYWORDS:

1-deoxy-d-xylulose 5-phosphate; 1-hydroxy-2-methyl-2-butenyl 4-diphosphate; Arabidopsis thaliana; Co-expression analysis; DXP; GGR; HMBPP; IPP; Isoprenoids; MEP; PDS; PIF5; PIFs; Phytochrome-interacting factor; bHLH; basic helix-loop-helix; geranylgeranyl reductase; isopentyl diphosphate; methyl-d-erythritol 5-phosphate; phytochrome-interacting factor 5; phytochrome-interacting factors; phytoene desaturase

PMID:
24342623
DOI:
10.1016/j.bbrc.2013.12.040
[Indexed for MEDLINE]

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