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Biochem Biophys Res Commun. 2014 Jan 17;443(3):858-63. doi: 10.1016/j.bbrc.2013.12.058. Epub 2013 Dec 14.

Cell adhesion and invasion inhibitory effect of an ovarian cancer targeting peptide selected via phage display in vivo.

Author information

1
College of Materials Science and Engineering, Sichuan University, Chengdu 610065, PR China.
2
College of Materials Science and Engineering, Sichuan University, Chengdu 610065, PR China. Electronic address: nic0700@scu.edu.cn.

Abstract

Organ-specific metastasis is of great importance since most of the cancer deaths are caused by spread of the primary cancer to distant sites. Therefore, targeted anti-metastases therapies are needed to prevent cancer cells from metastasizing to different organs. The phage clone pc3-1 displaying peptide WSGPGVWGASVK selected by phage display had been identified which have high binding efficiency and remarkable cell specificity to SK-OV-3 cells. In the present work, the effects of selected cell-binding phage and cognate peptide on the cell adhesion and invasion of targeted cells were investigated. Results showed that the adhesive ability of SK-OV-3 to extracellular matrix was inhibited by pc3-1 and peptide WSGPGVWGASVK, and pc3-1 blocked SK-OV-3 cells attachment more effective than the cognate peptide. The peptide WSGPGVWGASVK suppressed the cell number of SK-OV-3 that attached to HUVECs monolayer up to 24% and could block the spreading of the attaching cells. Forthermore, the cognate peptide could inhibit the invasion of SK-OV-3 significantly. The number of invaded SK-OV-3 cells and invaded SK-OV-3-activated HUVECs pretreated with peptide WSGPGVWGASVK was decreased by 24.3% and 36.6%, respectively. All these results suggested that peptide WSGPGVWGASVK might possess anti-metastasis against SK-OV-3 cells.

KEYWORDS:

Anti-metastasis; Cell adhesion; Cell invasion; Inhibitory effect; Ovarian cancer targeting peptide

PMID:
24342617
DOI:
10.1016/j.bbrc.2013.12.058
[Indexed for MEDLINE]
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