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Mol Cell Endocrinol. 2014 Mar 5;383(1-2):137-46. doi: 10.1016/j.mce.2013.12.005. Epub 2013 Dec 14.

Human white adipocytes express the cold receptor TRPM8 which activation induces UCP1 expression, mitochondrial activation and heat production.

Author information

1
Department of Medicine - DIMED, Clinica Medica 3, School of Medicine, University of Padova, Padova, Italy. Electronic address: marco.rossato@unipd.it.
2
Department of Medicine - DIMED, Clinica Medica 3, School of Medicine, University of Padova, Padova, Italy.
3
Department of Molecular Medicine, Section of Anatomy, School of Medicine, University of Padova, Padova, Italy.
4
Department of Animal Medicine, Production and Health, University of Padova, Padova, Italy.
5
Department of Biomedical Sciences, School of Medicine, University of Padova, Padova, Italy.
6
Department of Neurosciences, Plastic Surgery Clinic, University of Padova, Padova, Italy.

Abstract

Mammals possess two types of adipose tissue, white (WAT) and brown (BAT). The uncoupling protein 1 (UCP1) is a hallmark of BAT, being the pivotal player for cold-induced thermogenesis. WAT can acquire BAT characteristics with up-regulation of UCP1 after cold exposure or adrenergic stimulation. In the present study we demonstrated that human white adipocytes express the cold-sensing receptor TRPM8 which activation by menthol and icilin induced a rise in [Ca²⁺](i) and UCP1 expression, increased mitochondrial membrane potential, glucose uptake and heat production. The induction of "brown-like" phenotype in human white adipocytes after TRPM8 activation was supported by ultrastructural morphological changes of mitochondrial morphology and of their intracellular localization, with no modifications of the genes regulating mitochondrial biogenesis. In conclusion human white adipocytes express the cold receptor TRPM8 which activation induces their "browning" supporting a possible role of this receptor in the control of adipose tissue metabolism and body energy balance.

KEYWORDS:

Human adipocyte; Menthol; Mitochondria; TRPM8; UCP1

PMID:
24342393
DOI:
10.1016/j.mce.2013.12.005
[Indexed for MEDLINE]
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