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Anal Biochem. 1986 Nov 15;159(1):227-32.

A procedure for Northern blot analysis of native RNA.

Abstract

We describe a modification of the Northern technique that allows the detection of RNA either native and/or containing hidden breaks. We found that the highest sensitivity of the hybridization signals was obtained after denaturation of the RNA in the gel prior to its transfer onto a nylon membrane (GeneScreen) followed by uv irradiation. The sensitivity of the method using native RNA was found to be equivalent to that obtained with denatured RNA.

PMID:
2433961
DOI:
10.1016/0003-2697(86)90332-5
[Indexed for MEDLINE]

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