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Diabetes Metab Res Rev. 2014 Jul;30(5):424-34. doi: 10.1002/dmrr.2510.

Biomarker discovery study design for type 1 diabetes in The Environmental Determinants of Diabetes in the Young (TEDDY) study.

Collaborators (191)

Rewers M, Barriga K, Bautista K, Baxter J, Eisenbarth G, Frank N, Gesualdo P, Hoffman M, Ide L, Karban R, Liu E, Norris J, Waugh K, Samper-Imaz A, Steck A, She JX, Schatz D, Hopkins D, Steed L, Thomas J, Silvis K, Haller M, Shankar M, Sheehan E, Gardiner M, McIndoe R, Liu H, Nechtman J, Sharma A, Williams J, Foghis G, Anderson SW, Ziegler AG, Beyerlein A, Bonifacio E, Hummel M, Hummel S, Foterek K, Kersting M, Knopff A, Koletzko S, Peplow C, Roth R, Schenkel J, Stock J, Strauss E, Warncke K, Winkler C, Simell OG, Hyöty H, Ilonen J, Knip M, Koivu A, Koreasalo M, Kähönen M, Lönnrot M, Multasuo K, Mäntymäki E, Mykkänen J, Näntö-Salonen K, Niininen T, Nyblom M, Rautanen J, Riikonen A, Romo M, Simell A, Simell B, Simell T, Simell V, Sjöberg M, Stenius A, Toppari J, Varjonen E, Veijola R, Virtanen SM, Åkerlund M, Lernmark A, Agardh D, Andren-Aronsson C, Ask M, Bremer J, Carlsson UM, Cilio C, Ericson-Hallstrom E, Fransson L, Gard T, Gerardsson J, Hakansson R, Hansen M, Hansson G, Hyberg S, Johansen F, Jonasdottir B, Jonsson L, Larsson H, Lernmark B, Mansson-Martinez M, Markan M, Massadakis T, Melin J, Mestan Z, Nilsson A, Nilsson E, Rahmati K, Rosenquist A, Salami F, Jarvirova MS, Sibthorpe S, Sjoberg B, Swartling U, Trulsson E, Torn C, Wallin A, Wimar A, Aberg S, Hagopian WA, Yan X, Killian M, Crouch CC, Hay KM, Ayres S, Adams C, Bratrude B, Coughlin D, Fowler G, Franco C, Hammar C, Heaney D, Marcus P, Meyer A, Mulenga D, Scott E, Skidmore J, Stabbert J, Stepitova V, Williams N, Becker D, Franciscus M, Smith MD, Daftary A, Klein MB, Krischer JP, Abbondondolo M, Austin-Gonzalez S, Brown R, Burkhardt B, Butterworth M, Cuthbertson D, Eberhard C, Fiske S, Gowda V, Hadley D, Lane P, Lee HS, Liu S, Liu X, Lynch K, Malloy J, McCarthy C, McLeod W, Smith L, Smith S, Tamura R, Uusitalo U, Vehik K, Washington E, Yang J, Akolkar B, Bourcier K, Briese T, Johnson SB, Triplett E, Yu L, Miao D, Bingley P, Williams A, Chandler K, Rokni S, Long A, Boldison J, Butterly J, Broadhurst J, Carreno G, Curnock R, Easton P, Geoghan I, Goode J, Pearson J, Reed C, Ridewood S, Wyatt R.

Abstract

AIMS:

The Environmental Determinants of Diabetes in the Young planned biomarker discovery studies on longitudinal samples for persistent confirmed islet cell autoantibodies and type 1 diabetes using dietary biomarkers, metabolomics, microbiome/viral metagenomics and gene expression.

METHODS:

This article describes the details of planning The Environmental Determinants of Diabetes in the Young biomarker discovery studies using a nested case-control design that was chosen as an alternative to the full cohort analysis. In the frame of a nested case-control design, it guides the choice of matching factors, selection of controls, preparation of external quality control samples and reduction of batch effects along with proper sample allocation.

RESULTS AND CONCLUSION:

Our design is to reduce potential bias and retain study power while reducing the costs by limiting the numbers of samples requiring laboratory analyses. It also covers two primary end points (the occurrence of diabetes-related autoantibodies and the diagnosis of type 1 diabetes). The resulting list of case-control matched samples for each laboratory was augmented with external quality control samples.

KEYWORDS:

TEDDY; batch effects; biomarker discovery; nested case-control design; type 1 diabetes

PMID:
24339168
PMCID:
PMC4058423
DOI:
10.1002/dmrr.2510
[Indexed for MEDLINE]
Free PMC Article

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