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Cell Mol Neurobiol. 2014 Mar;34(2):289-96. doi: 10.1007/s10571-013-0013-x. Epub 2013 Dec 13.

DRG axon elongation and growth cone collapse rate induced by Sema3A are differently dependent on NGF concentration.

Author information

1
Biophysical Research Group, Biology Department, Vytautas Magnus University, Kaunas, Lithuania.

Abstract

Regeneration of embryonic and adult dorsal root ganglion (DRG) sensory axons is highly impeded when they encounter neuronal growth cone-collapsing factor semaphorin3A (Sema3A). On the other hand, increasing evidence shows that DRG axon's regeneration can be stimulated by nerve growth factor (NGF). In this study, we aimed to evaluate whether increased NGF concentrations can counterweight Sema3A-induced inhibitory responses in 15-day-old mouse embryo (E15) DRG axons. The DRG explants were grown in Neurobasal-based medium with different NGF concentrations ranging from 0 to 100 ng/mL and then treated with Sema3A at constant 10 ng/mL concentration. To evaluate interplay between NGF and Sema3A number of DRG axons, axon outgrowth distance and collapse rate were measured. We found that the increased NGF concentrations abolish Sema3A-induced inhibitory effect on axon outgrowth, while they have no effect on Sema3A-induced collapse rate.

PMID:
24338202
DOI:
10.1007/s10571-013-0013-x
[Indexed for MEDLINE]

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