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Phys Med Biol. 2014 Jan 6;59(1):189-201. doi: 10.1088/0031-9155/59/1/189. Epub 2013 Dec 13.

Three dimensional visualization of engineered bone and soft tissue by combined x-ray micro-diffraction and phase contrast tomography.

Author information

1
Istituto di Fotonica e Nanotecnologie - CNR, via Cineto Romano 42, I-00156 Roma, Italy.

Abstract

Computed x-ray phase contrast micro-tomography is the most valuable tool for a three dimensional (3D) and non destructive analysis of the tissue engineered bone morphology. We used a Talbot interferometer installed at SYRMEP beamline of the ELETTRA synchrotron (Trieste, Italy) for a precise 3D reconstruction of both bone and soft connective tissue, regenerated in vivo within a porous scaffold. For the first time the x-ray tomographic reconstructions have been combined with x-ray scanning micro-diffraction measurement on the same sample, in order to give an exhaustive identification of the different tissues participating to the biomineralization process. As a result, we were able to investigate in detail the different densities in the tissues, distinguishing the 3D organization of the amorphous calcium phosphate from the collagen matrix. Our experimental approach allows for a deeper understanding of the role of collagen matrix in the organic-mineral transition, which is a crucial issue for the development of new bio-inspired composites.

PMID:
24334371
DOI:
10.1088/0031-9155/59/1/189
[Indexed for MEDLINE]

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