Synthesis of a novel molecularly imprinted organic-inorganic hybrid polymer for the selective isolation and determination of fluoroquinolones in tilapia

J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Jan 15:945-946:127-34. doi: 10.1016/j.jchromb.2013.11.040. Epub 2013 Nov 25.

Abstract

A novel molecularly imprinted organic-inorganic hybrid polymer (MI-MAA/APTS) based on a dummy molecular imprinting technique and an organic-inorganic hybrid material technique was synthesised and used as a sorbent in solid-phase extraction for the selective isolation and determination of ofloxacin (OFL), lomefloxacin (LOM), and ciprofloxacin (CIP) in tilapia samples. The MI-MAA/APTS sorbent was prepared from 3-aminopropyltriethoxysilanes (APTS) as an inorganic source and methacrylic acid (MAA) as an organic source and exhibited high mechanical strength and special affinities to the analytes. A comparison of MI-MAA/APTS with other conventional sorbents (C18 and HLB) showed that MI-MAA/APTS displayed good selectivity and affinity for OFL, LOM, and CIP, and the recoveries of the analytes at three spiked levels were in the range of 85.1-101.0%, with the relative standard deviations ≤5.1%. The presented MI-MAA/APTS-SPE-HPLC method could be potentially applied to the determination of fluoroquinolones (FQs) in complex fish samples.

Keywords: Fluoroquinolones; Molecular imprinting; Organic–inorganic hybrid materials; Solid-phase extraction; Tilapia.

Publication types

  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Animals
  • Anti-Bacterial Agents / analysis*
  • Anti-Bacterial Agents / isolation & purification
  • Chromatography, High Pressure Liquid / methods
  • Fluoroquinolones / analysis*
  • Fluoroquinolones / isolation & purification
  • Limit of Detection
  • Molecular Imprinting / methods*
  • Polymers / chemistry
  • Solid Phase Extraction / methods
  • Tilapia / metabolism*

Substances

  • Anti-Bacterial Agents
  • Fluoroquinolones
  • Polymers