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Cancer Lett. 2014 Apr 28;346(1):74-83. doi: 10.1016/j.canlet.2013.12.011. Epub 2013 Dec 12.

Far-infrared radiation inhibits proliferation, migration, and angiogenesis of human umbilical vein endothelial cells by suppressing secretory clusterin levels.

Author information

1
Department of Molecular Medicine, Ewha Womans University School of Medicine, Seoul, Republic of Korea.
2
Division of Physical Metrology, Korea Research Institute of Standards and Science (KRISS), Daejeon, Republic of Korea.
3
Department of Internal Medicine, Kyungpook National University School of Medicine, Daegu, Republic of Korea.
4
Department of Molecular Medicine, Ewha Womans University School of Medicine, Seoul, Republic of Korea. Electronic address: inhojo@ewha.ac.kr.

Abstract

Far-infrared (FIR) radiation is known to lessen the risk of angiogenesis-related diseases including cancer. Because deficiency of secretory clusterin (sCLU) has been reported to inhibit angiogenesis of endothelial cells (EC), we investigated using human umbilical vein EC (HUVEC) whether sCLU mediates the inhibitory effects of FIR radiation. Although FIR radiation ranging 3-25μm wavelength at room temperature for 60min did not alter EC viability, further incubation in the culture incubator (at 37°C under 5% CO2) after radiation significantly inhibited EC proliferation, in vitro migration, and tube formation in a time-dependent manner. Under these conditions, we found decreased sCLU mRNA and protein expression in HUVEC and decreased sCLU protein secreted in culture medium. Expectedly, the replacement of control culture medium with the FIR-irradiated conditioned medium significantly decreased wound closure and tube formation of HUVEC, and vice versa. Furthermore, neutralization of sCLU with anti-sCLU antibody also mimicked all observed inhibitory effects of FIR radiation. Moreover, treatment with recombinant human sCLU protein completely reversed the inhibitory effects of FIR radiation on EC migration and angiogenesis. Lastly, vascular endothelial growth factor also increased sCLU secretion in the culture medium, and wound closure and tube formation of HUVEC, which were significantly reduced by FIR radiation. Our results demonstrate a novel mechanism by which FIR radiation inhibits the proliferation, migration, and angiogenesis of HUVEC, via decreasing sCLU.

KEYWORDS:

Angiogenesis; Clusterin; Endothelial cell; Far-infrared radiation

PMID:
24334140
DOI:
10.1016/j.canlet.2013.12.011
[Indexed for MEDLINE]

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