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Mutat Res. 2014 Jan;759:9-15. doi: 10.1016/j.mrfmmm.2013.11.006. Epub 2013 Dec 12.

Low concentration of exogenous carbon monoxide protects mammalian cells against proliferation induced by radiation-induced bystander effect.

Author information

1
Center of Medical Physics and Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, People's Republic of China.
2
Department of Physics and Materials Science, City University of Hong Kong, Tat Chee Avenue, Kowloon Tong, Hong Kong; Center of Medical Physics and Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, People's Republic of China.
3
Center of Medical Physics and Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, People's Republic of China. Electronic address: hanw@hfcas.cn.

Abstract

Radiation-induced bystander effect (RIBE) has been proposed to have tight relationship with the irradiation-caused secondary cancers beyond the irradiation-treated area after radiotherapy. Our previous studies demonstrated a protective effect of low concentration carbon monoxide (CO) on the genotoxicity of RIBE after α-particle irradiation. In the present work, a significant inhibitory effect of low-dose exogenous CO, generated by tricarbonyldichlororuthenium (II) dimer [CO-releasing molecule (CORM-2)], on both RIBE-induced proliferation and chromosome aberration was observed. Further studies on the mechanism revealed that the transforming growth factor β1/nitric oxide (NO) signaling pathway, which mediated RIBE signaling transduction, could be modulated by CO involved in the protective effects. Considering the potential of exogenous CO in clinical applications and its protective effect on RIBE, the present work aims to provide a foundation for potential application of CO in radiotherapy.

KEYWORDS:

2′,7′-difluorofluorescein diacetate; 4-amini-5-methylamino-2′,7′-difluorofluorescein diacetate; AG; CBMN; CHO; CO; CO releasing molecule; CORM; COX-2; Carbon monoxide; Chinese hamster ovary; Chromosome aberration; DAF-FM; DCF; DMSO; DNA double-strand breaks; DSBs; HO; MN; NADPH; NF-κB; NO; NOS; NPB; Proliferation; RCG; RIBE; ROS; Radiation-induced bystander effect; TGF-β1; TNF-α; aminoguanidine hemisulfate salt; carbon monoxide; cyclooxygenase-2; cytokinesis-B blocked micronucleus; dimethyl sulfoxide; heme oxygenase; micronucleus; nicotinamide adenine dinucleotide phosphate; nitric oxide; nitric oxide synthase; nuclear factor-κB; nucleoplasmic bridges; radiation induced bystander effect; reactive oxygen species; relative cell growth; transforming growth factor-β1; tumor necrosis factor-α

PMID:
24333162
DOI:
10.1016/j.mrfmmm.2013.11.006
[Indexed for MEDLINE]

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