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Vet Parasitol. 2014 Feb 24;200(1-2):225-8. doi: 10.1016/j.vetpar.2013.11.018. Epub 2013 Nov 26.

In vitro and in vivo effects of neem tree (Azadirachta indica A. Juss) products on larvae of the sheep nose bot fly (Oestrus ovis L. Díptera: Oestridae).

Author information

1
Departamento de Zootecnia, Universidad Autónoma de Baja California Sur, Km. 5.5 carr. al Sur. A.P. 19-B, C.P. 23080 La Paz, B.C.S., Mexico.
2
Centro de Investigaciones Biológicas del Noroeste, S.C. Instituto Politécnico Nacional 195, Playa Palo de Santa Rita Sur, C.P. 23096 La Paz, B.C.S., Mexico.
3
Ecole Nationale Vétérinaire de Toulouse, 23 Chemin des Capelles, 31076, Toulouse Cedex 03, France.
4
Centro de Investigaciones Biológicas del Noroeste, S.C. Instituto Politécnico Nacional 195, Playa Palo de Santa Rita Sur, C.P. 23096 La Paz, B.C.S., Mexico. Electronic address: eangulo@cibnor.mx.

Abstract

Two studies were carried out in order to test the effects of neem tree extracts (Azadirachta indica A. Juss) on sheep bot fly larvae (Oestrus ovis L. Diptera: Oestridae). First, aqueous extracts from neem seeds (ASNE) at 0, 5 y 10% (w/v) concentrations were tested on larval mortality in vitro. In a second study, the effect of oral administration with neem seed meal (0, 100 y 200mg/kg) and neem leaves (1% of diet) on number of larvae found at necropsy and larval development was evaluated in experimentally O. ovis-infected sheep. Results in Experiment 1 showed a significant (P<0.05) effect of ASNE on time to L1 mortality in a dosis-dependent manner. In Experiment 2, oral administration of seeds or leaves did not affect the number of larvae found at necropsy of the sheep, but interfered with larval development and there was a tendency to reduce larval weight at the end of the infection period (55d).

KEYWORDS:

Larval development; Larval mortality; Neem (Azadirachta indica A. Juss); O. ovis; Sheep

PMID:
24332964
DOI:
10.1016/j.vetpar.2013.11.018
[Indexed for MEDLINE]

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