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Carbohydr Res. 2014 May 7;389:12-7. doi: 10.1016/j.carres.2013.11.008. Epub 2013 Nov 17.

Carbohydrate-functionalized collagen matrices: design and characterization of a novel neoglycosylated biomaterial.

Author information

  • 1Department of Biotechnology and Biosciences, Università degli Studi Milano-Bicocca, Piazza della Scienza 2, Milan, Italy.
  • 2Dipartimento di Elettronica, Informazione e Bioingegneria, Politecnico di Milano, Piazza Leonardo da Vinci 32, Milan, Italy.
  • 3Dipartimento di Scienze Chirurgiche e Morfologiche, Università degli Studi dell'Insubria, Via Guicciardini 9, 21100 Varese, Italy.
  • 4Department of Nanomedicine, The Methodist Hospital Research Institute, 6670 Bertner Ave, Houston, TX 77030, USA; Pain Therapy Service, Fondazione IRCCS Policlinico San Matteo, via Golgi, 19, 27100 Pavia, Italy.
  • 5Department of Biotechnology and Biosciences, Università degli Studi Milano-Bicocca, Piazza della Scienza 2, Milan, Italy. Electronic address: laura.cipolla@unimib.it.

Abstract

Collagen matrices have been neoglycosylated with lactose by reductive amination at lysine side chains. AFM analysis highlights that the chemical does not affect molecular assembly into fibrils. Moreover, ELLA biochemical assays show that the glycan moiety is efficiently exposed on the matrix surface for receptor recognition.

KEYWORDS:

AFM; Collagen; Lactose; Lectins; Neoglycosylation; Reductive amination

PMID:
24332940
DOI:
10.1016/j.carres.2013.11.008
[PubMed - indexed for MEDLINE]
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