Format

Send to

Choose Destination
See comment in PubMed Commons below
Diagn Microbiol Infect Dis. 2014 Mar;78(3):201-12. doi: 10.1016/j.diagmicrobio.2013.11.007. Epub 2013 Nov 15.

Multimer monitoring of CMV-specific T cells in research and in clinical applications.

Author information

1
Department of Hematology, Hemostasis, Oncology and Stem Cell Transplantation, Hannover Medical School (MHH), Hannover, Germany; Integrated Research and Treatment Center Transplantation (IFB-Tx), Hannover, Germany; German Centre for Infection Research (DZIF), Partnerside Hannover-Braunschweig, Germany. Electronic address: borchers.sylvia@mh-hannover.de.
2
Department of Hematology, Hemostasis, Oncology and Stem Cell Transplantation, Hannover Medical School (MHH), Hannover, Germany. Electronic address: ogonek.justyna@mh-hannover.de.
3
Department of Hematology, Hemostasis, Oncology and Stem Cell Transplantation, Hannover Medical School (MHH), Hannover, Germany; Integrated Research and Treatment Center Transplantation (IFB-Tx), Hannover, Germany; German Centre for Infection Research (DZIF), Partnerside Hannover-Braunschweig, Germany. Electronic address: varanasi.pavankumar@mh-hannover.de.
4
Institute of Transfusion Medicine, MHH, Hannover, Germany. Electronic address: tischer.sabine@mh-hannover.de.
5
Pediatric Hematology and Oncology, Johann Wolfgang Goethe-University, Frankfurt, Germany. Electronic address: Melanie.Bremm@kgu.de.
6
Integrated Research and Treatment Center Transplantation (IFB-Tx), Hannover, Germany; Institute of Transfusion Medicine, MHH, Hannover, Germany. Electronic address: eiz-vesper.britta@mh-hannover.de.
7
Integrated Research and Treatment Center Transplantation (IFB-Tx), Hannover, Germany; Institute for Cellular Therapeutics, MHH, Hannover, Germany. Electronic address: koehl.ulrike@mh-hannover.de.
8
Department of Hematology, Hemostasis, Oncology and Stem Cell Transplantation, Hannover Medical School (MHH), Hannover, Germany; Integrated Research and Treatment Center Transplantation (IFB-Tx), Hannover, Germany; German Centre for Infection Research (DZIF), Partnerside Hannover-Braunschweig, Germany. Electronic address: mischak-weissinger.eva@mh-hannover.de.

Abstract

Multimer monitoring has become a standard technique for detection of antigen-specific T cells. The term "multimer" refers to a group of reagents based on the multimerisation of molecules in order to raise avidity and thus stabilize binding to their ligand. Multimers for detection of antigen-specific T-cell responses are based on major histocompatibility complex class I peptide complexes. Multimer staining enables fast and direct visualization of antigen-specific T cells; thus, it is widely applied to assess antiviral immunity, e.g., monitor patients in vaccination trials or confirm purity of cell products for adoptive transfer. Assessment of T-cell immunity against persistent pathogens like cytomegalovirus (CMV) is of major importance in immunosuppressed patients. Recent advancements of multimers facilitate reversible labeling and allow isolation of epitope-specific T cells for adoptive transfer. Here, we give an overview on the different multimers and their applications, with an emphasis on CMV-specific T-cell responses.

KEYWORDS:

Cytomegalovirus; Histamer; Infectious complications; Multimer; Pentamer; Stem cell transplantation; Streptamer; T cell; Tetramer

[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center