Format

Send to

Choose Destination
J Plant Physiol. 2014 Jan 15;171(2):136-9. doi: 10.1016/j.jplph.2013.09.020. Epub 2013 Nov 20.

Quantitative analysis of herbivore-induced cytosolic calcium by using a Cameleon (YC 3.6) calcium sensor in Arabidopsis thaliana.

Author information

1
Plant Physiology Unit, Dept. Life Sciences and Systems Biology, University of Turin, Italy. Electronic address: francesca.verrillo@unito.it.
2
Plant Physiology Unit, Dept. Life Sciences and Systems Biology, University of Turin, Italy. Electronic address: andrea.occhipinti@unito.it.
3
Research and Innovation Center Biology and Genomic of Fruit Plants, Fondazione Edmund Mach FEM San Michele all'Adige (TN), Italy. Electronic address: chidubt@gmail.com.
4
Plant Physiology Unit, Dept. Life Sciences and Systems Biology, University of Turin, Italy. Electronic address: massimo.maffei@unito.it.

Abstract

Ca(2+) is a key player in plant cell responses to biotic and abiotic stress. Owing to the central role of cytosolic Ca(2+) ([Ca(2+)]cyt) during early signaling and the need for precise determination of [Ca(2+)]cyt variations, we used a Cameleon YC 3.6 reporter protein expressed in Arabidopsis thaliana to quantify [Ca(2+)]cyt variations upon leaf mechanical damage (MD), herbivory by 3rd and 5th instar larvae of Spodoptera littoralis and S. littoralis oral secretions (OS) applied to MD. YC 3.6 allowed a clear distinction between MD and herbivory and discriminated between the two larvae instars. To our knowledge this is the first report of quantitative [Ca(2+)]cyt determination upon herbivory using a Cameleon calcium sensor.

KEYWORDS:

Arabidopsis thaliana; Calcium signaling; Cameleon YC 3.6; Herbivory; Spodoptera littoralis

PMID:
24331428
DOI:
10.1016/j.jplph.2013.09.020
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science Icon for University of Turin Instituional Repository AperTO
Loading ...
Support Center