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Obes Res Clin Pract. 2011 Jan-Mar;5(1):e1-e78. doi: 10.1016/j.orcp.2010.12.002.

The use of adipose tissue-conditioned media to demonstrate the differential effects of fat depots on insulin-stimulated glucose uptake in a skeletal muscle cell line.

Author information

1
Discipline of Medicine, University of Adelaide, Adelaide, SA 5005, Australia. Electronic address:yan.lam@sydney.edu.au.
2
Discipline of Medicine, University of Adelaide, Adelaide, SA 5005, Australia.
3
School of Biomedical and Health Sciences, Victoria University, Melbourne, VIC 3000, Australia.
4
CSIRO Food and Nutritional Sciences, Adelaide, SA 5000, Australia.
5
Discipline of Surgery, University of Adelaide, Adelaide, SA 5005, Australia.

Abstract

SUMMARY:

We aimed to study the depot-specific effect of adipose tissue on insulin sensitivity of skeletal muscle in vitro. Adipose tissue-conditioned medium (CM) was generated from visceral and subcutaneous fat from obese subjects. CM from visceral as compared to subcutaneous fat had higher concentrations of interleukin (IL)-6 (15-fold; P < 0.05) and IL-8 (8-fold; P < 0.05). CM from visceral fat (1:128 dilution) reduced insulin-stimulated glucose uptake in L6 myotubes by 19% (P < 0.05), an effect mediated by a nuclear factor kappa B (NFκB)/mammalian target of rapamycin complex 1 (mTORC1)-dependent pathway and partially reversed by neutralizing IL-6. IL-6 at a concentration comparable to that in CM from visceral fat reduced insulin-stimulated glucose uptake by 53% (P < 0.05), an effect abolished by inhibiting NFκB or mTORC1. We demonstrated the utility of the CM-myotube system and identified IL-6 as a major cytokine mediating visceral fat-induced muscle insulin resistance.:

PMID:
24331010
DOI:
10.1016/j.orcp.2010.12.002
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