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Biomacromolecules. 2014 Jan 13;15(1):276-82. doi: 10.1021/bm401532n. Epub 2013 Dec 26.

Development and characterization of gene silencing DNA cages.

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Department of Chemistry and Center for Self-Assembled Chemical Structures, McGill University , 801 Sherbrooke Street West, Montreal, Quebec H3A 0B8, Canada.


RNA interference (RNAi) is a powerful therapeutic strategy that induces gene silencing by targeting disease-causing mRNA and can lead to their removal through degradation pathways. The potential of RNAi is especially relevant in cancer therapy, as it can be designed to regulate the expression of genes involved in all stages of tumor development (initiation, growth, and metastasis). We have generated gene silencing 3D DNA prisms that integrate antisense oligonucleotide therapeutics at 1, 2, 4, and 6 positions. Synthesis of these structures is readily achieved and leads to the assembly of highly monodisperse and well-characterized structures. We have shown that antisense strands scaffolded on DNA cages can readily induce gene silencing in mammalian cells and maintain gene knockdown levels more effectively than single and double stranded controls through increased stability of bound antisense units.

[Indexed for MEDLINE]

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