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Sci Rep. 2013 Dec 11;3:3474. doi: 10.1038/srep03474.

Matrix identity and tractional forces influence indirect cardiac reprogramming.

Author information

1
Department of Biomedical Engineering, University of Michigan College of Engineering, Ann Arbor, Michigan, 48109, USA.

Abstract

Heart regeneration through in vivo cardiac reprogramming has been demonstrated as a possible regenerative strategy. While it has been reported that cardiac reprogramming in vivo is more efficient than in vitro, the influence of the extracellular microenvironment on cardiac reprogramming remains incompletely understood. This understanding is necessary to improve the efficiency of cardiac reprogramming in order to implement this strategy successfully. Here we have identified matrix identity and cell-generated tractional forces as key determinants of the dedifferentiation and differentiation stages during reprogramming. Cell proliferation, matrix mechanics, and matrix microstructure are also important, but play lesser roles. Our results suggest that the extracellular microenvironment can be optimized to enhance cardiac reprogramming.

PMID:
24326998
PMCID:
PMC3858798
DOI:
10.1038/srep03474
[Indexed for MEDLINE]
Free PMC Article

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