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Analyst. 2014 Feb 7;139(3):576-80. doi: 10.1039/c3an02104a.

Selective detection of complementarity-determining regions of monoclonal antibody by limiting protease access to the substrate: nano-surface and molecular-orientation limited proteolysis.

Author information

1
Life Science Research Center, SHIMADZU Corporation, Kanda-Nishiki-cho, Chiyoda-ku, Tokyo 101-8448, Japan. t-shima@shimadzu.co.jp.

Abstract

We report a novel method for the selective proteolysis by limiting protease access to the substrate, which we have named nano-surface and molecular-orientation limited (nSMOL) proteolysis. We focus on the identification of the Fab and quantitation of antibodies. This method successfully performed limited proteolysis on the Fab.

PMID:
24326404
DOI:
10.1039/c3an02104a
[Indexed for MEDLINE]

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